3700s atg5 rabbit polyclonal to atg5 (Cell Signaling Technology Inc)
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3700s Atg5 Rabbit Polyclonal To Atg5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 6738 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CYP2E1 protein was detected by chemiluminescent ECL Detection Kit (Thermo Fisher Scientific) in a photodocumentation system (G:BOX, Syngene, Cambridge, UK). .. Membranes were stripped with Restore TM Stripping Buffer (Thermo Fisher Scientific) for 15 minutes, washed, blocked again and incubated overnight at 4°C with Article Title: Metabolic Intervention Liposome Boosted Lung Cancer Radio-Immunotherapy via Hypoxia Amelioration and PD-L1 Restraint. Article Snippet: .. The PVDF membrane was closed with 5% skim milk and incubated with Article Title: Killing three birds with one stone: Multi-stage metabolic regulation mediated by clinically usable berberine liposome to overcome photodynamic immunotherapy resistance Article Snippet: Nowadays, photodynamic therapy (PDT) has become a novel effect modality for cancer therapy.. But, facing the extremely hypoxic tumor microenvironment, the efficacy of PDT is still impaired owing to the limited reactive oxygen species (ROS) production.. Moreover, after PDT, the overexpression of programmed cell death-ligand 1 (PD-L1) and indoleamine-2,3-dioxygenase-1 (IDO1) could trigger the following impaired T cell infiltration and the further lowered anti-tumor immunity mediated by T cells. Article Title: ITK overexpression enhances T cell cytotoxicity against DLBCL through the TCR-Ca 2+ -Calcineurin-NFAT-IFN-γ pathway Article Snippet: The proteins were then transferred to PVDF membranes (Millipore, USA, IPVH00010). .. The membranes were blocked with 5% non-fat milk (BD, USA, 232,100) in TBST buffer (20 mM Tris–HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.6) for 1–2 h. The membranes were incubated overnight at 4 °C with rabbit anti-mouse ITK antibody (Cell Signaling Technology, USA, #3769) diluted 1:1000 and dsDNA Assay:Article Title: Herba Origani alleviated DSS-induced ulcerative colitis in mice through remolding gut microbiota to regulate bile acid and short-chain fatty acid metabolisms. Article Snippet: This study aimed to investigate the protective effect of Herba Origani, the dried whole herb of Origanum vulgare L., on dextran sodium sulfate (DSS)-induced ulcerative colitis in mice and explore its mechanisms of action through analyzing the intestinal microbiota in cecum contents and metabolites in colonic tissues.. HOEP alleviated colitis symptoms, colonic inflammation and pathological injury as well as repaired intestinal barrier function in DSS-induced UC mice.. The intestinal microbiota analysis showed that HOEP restored the gut microbiota dysbiosis in DSS-treated mice by increasing the alpha diversity of the intestinal microbiota, increasing the abundance of the Bacteroidota community and adjusting short-chain fatty acids (SCFAs), which maintain mucosal immunity and intestinal barrier. Enzyme-linked Immunosorbent Assay:Article Title: Herba Origani alleviated DSS-induced ulcerative colitis in mice through remolding gut microbiota to regulate bile acid and short-chain fatty acid metabolisms. Article Snippet: This study aimed to investigate the protective effect of Herba Origani, the dried whole herb of Origanum vulgare L., on dextran sodium sulfate (DSS)-induced ulcerative colitis in mice and explore its mechanisms of action through analyzing the intestinal microbiota in cecum contents and metabolites in colonic tissues.. HOEP alleviated colitis symptoms, colonic inflammation and pathological injury as well as repaired intestinal barrier function in DSS-induced UC mice.. 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Article Snippet: This study aimed to investigate the protective effect of Herba Origani, the dried whole herb of Origanum vulgare L., on dextran sodium sulfate (DSS)-induced ulcerative colitis in mice and explore its mechanisms of action through analyzing the intestinal microbiota in cecum contents and metabolites in colonic tissues.. HOEP alleviated colitis symptoms, colonic inflammation and pathological injury as well as repaired intestinal barrier function in DSS-induced UC mice.. The intestinal microbiota analysis showed that HOEP restored the gut microbiota dysbiosis in DSS-treated mice by increasing the alpha diversity of the intestinal microbiota, increasing the abundance of the Bacteroidota community and adjusting short-chain fatty acids (SCFAs), which maintain mucosal immunity and intestinal barrier. Bicinchoninic Acid Protein Assay:Article Title: Herba Origani alleviated DSS-induced ulcerative colitis in mice through remolding gut microbiota to regulate bile acid and short-chain fatty acid metabolisms. Article Snippet: This study aimed to investigate the protective effect of Herba Origani, the dried whole herb of Origanum vulgare L., on dextran sodium sulfate (DSS)-induced ulcerative colitis in mice and explore its mechanisms of action through analyzing the intestinal microbiota in cecum contents and metabolites in colonic tissues.. HOEP alleviated colitis symptoms, colonic inflammation and pathological injury as well as repaired intestinal barrier function in DSS-induced UC mice.. The intestinal microbiota analysis showed that HOEP restored the gut microbiota dysbiosis in DSS-treated mice by increasing the alpha diversity of the intestinal microbiota, increasing the abundance of the Bacteroidota community and adjusting short-chain fatty acids (SCFAs), which maintain mucosal immunity and intestinal barrier. Stripping Membranes:Article Title: Aedes aegypti mosquito saliva ameliorates acetaminophen-induced liver injury in mice Article Snippet: Antibody excess was removed by washing the membranes several times in TBS-T. CYP2E1 protein was detected by chemiluminescent ECL Detection Kit (Thermo Fisher Scientific) in a photodocumentation system (G:BOX, Syngene, Cambridge, UK). .. 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