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3700s atg5 rabbit polyclonal to atg5  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc 3700s atg5 rabbit polyclonal to atg5
    3700s Atg5 Rabbit Polyclonal To Atg5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 6738 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+mouse+%CE%B2+actin+antibody/beta-Actin+Mouse+mAb/pm41770818-302-14-12
    Average 99 stars, based on 6738 article reviews
    3700s atg5 rabbit polyclonal to atg5 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Podoplanin Drives Motility of Active Macrophage via Regulating Filamin C During Helicobacter pylori Infection
    Article Snippet: .. Cells were incubated with rabbit anti-mouse-β-actin antibody (Cell Signaling Technology, Beverly, MA) for 1 h followed by incubation with secondary antibodies Alexa Fluor 555-conjugated anti-rabbit IgG (H+L) F(ab’) 2 (Cell Signaling Technology). ..

    Article Title: ITK overexpression enhances T cell cytotoxicity against DLBCL through the TCR-Ca 2+ -Calcineurin-NFAT-IFN-γ pathway.
    Article Snippet: The proteins were then transferred to PVDF membranes (Millipore, USA, IPVH00010). .. The membranes were blocked with 5% non-fat milk (BD, USA, 232,100) in TBST buffer (20 mM Tris–HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.6) for 1–2 h. The membranes were incubated overnight at 4 °C with rabbit anti-mouse ITK antibody (Cell Signaling Technology, USA, #3769) diluted 1:1000 and rabbit anti-mouse β-actin antibody (Cell Signaling Technology, USA, #4970) diluted 1:2000. .. HRP-labeled goat anti-rabbit secondary antibody (Cell Signaling Technology, USA, #7074) diluted 1:5000 was added and incubated at room temperature for 1–2 h. After washing with TBST, the membranes were developed using a chemiluminescent substrate (Thermo Fisher Scientific, USA, 34,580) and exposed on a chemiluminescent imaging system (Bio-Rad, USA, ChemiDoc MP).

    Article Title: Aedes aegypti mosquito saliva ameliorates acetaminophen-induced liver injury in mice
    Article Snippet: Antibody excess was removed by washing the membranes several times in TBS-T. CYP2E1 protein was detected by chemiluminescent ECL Detection Kit (Thermo Fisher Scientific) in a photodocumentation system (G:BOX, Syngene, Cambridge, UK). .. Membranes were stripped with Restore TM Stripping Buffer (Thermo Fisher Scientific) for 15 minutes, washed, blocked again and incubated overnight at 4°C with rabbit anti-mouse β-actin antibody (Cell Signaling Technology). .. Membranes were washed and incubated for 1 hour with anti-rabbit horseradish peroxidase-conjugated antibody (Cell Signaling Technology), and β-actin bands were visualized as described earlier.

    Article Title: Metabolic Intervention Liposome Boosted Lung Cancer Radio-Immunotherapy via Hypoxia Amelioration and PD-L1 Restraint.
    Article Snippet: .. The PVDF membrane was closed with 5% skim milk and incubated with rabbit anti-mouse β-actin antibody (1:5000, affinity), rabbit anti-mouse AMPK antibody (1:1000, CST), rabbit anti-mouse p-AMPK antibody (1:1000, CST), and rabbit anti-mouse CD274 antibody (1:1000, affinity), respectively, at 4 °C overnight. .. After 24 h, they were co-incubated with secondary antibodies at room temperature for another 2 h. Finally, the fluorescence chemiluminescence gel imaging system (Peiqing Science and Technology) was used to obtain the image of protein bands.

    Article Title: Killing three birds with one stone: Multi-stage metabolic regulation mediated by clinically usable berberine liposome to overcome photodynamic immunotherapy resistance
    Article Snippet: Nowadays, photodynamic therapy (PDT) has become a novel effect modality for cancer therapy.. But, facing the extremely hypoxic tumor microenvironment, the efficacy of PDT is still impaired owing to the limited reactive oxygen species (ROS) production.. Moreover, after PDT, the overexpression of programmed cell death-ligand 1 (PD-L1) and indoleamine-2,3-dioxygenase-1 (IDO1) could trigger the following impaired T cell infiltration and the further lowered anti-tumor immunity mediated by T cells.

    Article Title: ITK overexpression enhances T cell cytotoxicity against DLBCL through the TCR-Ca 2+ -Calcineurin-NFAT-IFN-γ pathway
    Article Snippet: The proteins were then transferred to PVDF membranes (Millipore, USA, IPVH00010). .. The membranes were blocked with 5% non-fat milk (BD, USA, 232,100) in TBST buffer (20 mM Tris–HCl, 150 mM NaCl, 0.1% Tween 20, pH 7.6) for 1–2 h. The membranes were incubated overnight at 4 °C with rabbit anti-mouse ITK antibody (Cell Signaling Technology, USA, #3769) diluted 1:1000 and rabbit anti-mouse β-actin antibody (Cell Signaling Technology, USA, #4970) diluted 1:2000. .. HRP-labeled goat anti-rabbit secondary antibody (Cell Signaling Technology, USA, #7074) diluted 1:5000 was added and incubated at room temperature for 1–2 h. After washing with TBST, the membranes were developed using a chemiluminescent substrate (Thermo Fisher Scientific, USA, 34,580) and exposed on a chemiluminescent imaging system (Bio-Rad, USA, ChemiDoc MP).

    dsDNA Assay:

    Article Title: Herba Origani alleviated DSS-induced ulcerative colitis in mice through remolding gut microbiota to regulate bile acid and short-chain fatty acid metabolisms.
    Article Snippet: This study aimed to investigate the protective effect of Herba Origani, the dried whole herb of Origanum vulgare L., on dextran sodium sulfate (DSS)-induced ulcerative colitis in mice and explore its mechanisms of action through analyzing the intestinal microbiota in cecum contents and metabolites in colonic tissues.. HOEP alleviated colitis symptoms, colonic inflammation and pathological injury as well as repaired intestinal barrier function in DSS-induced UC mice.. The intestinal microbiota analysis showed that HOEP restored the gut microbiota dysbiosis in DSS-treated mice by increasing the alpha diversity of the intestinal microbiota, increasing the abundance of the Bacteroidota community and adjusting short-chain fatty acids (SCFAs), which maintain mucosal immunity and intestinal barrier.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Herba Origani alleviated DSS-induced ulcerative colitis in mice through remolding gut microbiota to regulate bile acid and short-chain fatty acid metabolisms.
    Article Snippet: This study aimed to investigate the protective effect of Herba Origani, the dried whole herb of Origanum vulgare L., on dextran sodium sulfate (DSS)-induced ulcerative colitis in mice and explore its mechanisms of action through analyzing the intestinal microbiota in cecum contents and metabolites in colonic tissues.. HOEP alleviated colitis symptoms, colonic inflammation and pathological injury as well as repaired intestinal barrier function in DSS-induced UC mice.. The intestinal microbiota analysis showed that HOEP restored the gut microbiota dysbiosis in DSS-treated mice by increasing the alpha diversity of the intestinal microbiota, increasing the abundance of the Bacteroidota community and adjusting short-chain fatty acids (SCFAs), which maintain mucosal immunity and intestinal barrier.

    Lysis:

    Article Title: Herba Origani alleviated DSS-induced ulcerative colitis in mice through remolding gut microbiota to regulate bile acid and short-chain fatty acid metabolisms.
    Article Snippet: This study aimed to investigate the protective effect of Herba Origani, the dried whole herb of Origanum vulgare L., on dextran sodium sulfate (DSS)-induced ulcerative colitis in mice and explore its mechanisms of action through analyzing the intestinal microbiota in cecum contents and metabolites in colonic tissues.. HOEP alleviated colitis symptoms, colonic inflammation and pathological injury as well as repaired intestinal barrier function in DSS-induced UC mice.. The intestinal microbiota analysis showed that HOEP restored the gut microbiota dysbiosis in DSS-treated mice by increasing the alpha diversity of the intestinal microbiota, increasing the abundance of the Bacteroidota community and adjusting short-chain fatty acids (SCFAs), which maintain mucosal immunity and intestinal barrier.

    SDS Page:

    Article Title: Herba Origani alleviated DSS-induced ulcerative colitis in mice through remolding gut microbiota to regulate bile acid and short-chain fatty acid metabolisms.
    Article Snippet: This study aimed to investigate the protective effect of Herba Origani, the dried whole herb of Origanum vulgare L., on dextran sodium sulfate (DSS)-induced ulcerative colitis in mice and explore its mechanisms of action through analyzing the intestinal microbiota in cecum contents and metabolites in colonic tissues.. HOEP alleviated colitis symptoms, colonic inflammation and pathological injury as well as repaired intestinal barrier function in DSS-induced UC mice.. The intestinal microbiota analysis showed that HOEP restored the gut microbiota dysbiosis in DSS-treated mice by increasing the alpha diversity of the intestinal microbiota, increasing the abundance of the Bacteroidota community and adjusting short-chain fatty acids (SCFAs), which maintain mucosal immunity and intestinal barrier.

    Bicinchoninic Acid Protein Assay:

    Article Title: Herba Origani alleviated DSS-induced ulcerative colitis in mice through remolding gut microbiota to regulate bile acid and short-chain fatty acid metabolisms.
    Article Snippet: This study aimed to investigate the protective effect of Herba Origani, the dried whole herb of Origanum vulgare L., on dextran sodium sulfate (DSS)-induced ulcerative colitis in mice and explore its mechanisms of action through analyzing the intestinal microbiota in cecum contents and metabolites in colonic tissues.. HOEP alleviated colitis symptoms, colonic inflammation and pathological injury as well as repaired intestinal barrier function in DSS-induced UC mice.. The intestinal microbiota analysis showed that HOEP restored the gut microbiota dysbiosis in DSS-treated mice by increasing the alpha diversity of the intestinal microbiota, increasing the abundance of the Bacteroidota community and adjusting short-chain fatty acids (SCFAs), which maintain mucosal immunity and intestinal barrier.

    Stripping Membranes:

    Article Title: Aedes aegypti mosquito saliva ameliorates acetaminophen-induced liver injury in mice
    Article Snippet: Antibody excess was removed by washing the membranes several times in TBS-T. CYP2E1 protein was detected by chemiluminescent ECL Detection Kit (Thermo Fisher Scientific) in a photodocumentation system (G:BOX, Syngene, Cambridge, UK). .. Membranes were stripped with Restore TM Stripping Buffer (Thermo Fisher Scientific) for 15 minutes, washed, blocked again and incubated overnight at 4°C with rabbit anti-mouse β-actin antibody (Cell Signaling Technology). .. Membranes were washed and incubated for 1 hour with anti-rabbit horseradish peroxidase-conjugated antibody (Cell Signaling Technology), and β-actin bands were visualized as described earlier.

    Membrane:

    Article Title: Metabolic Intervention Liposome Boosted Lung Cancer Radio-Immunotherapy via Hypoxia Amelioration and PD-L1 Restraint.
    Article Snippet: .. The PVDF membrane was closed with 5% skim milk and incubated with rabbit anti-mouse β-actin antibody (1:5000, affinity), rabbit anti-mouse AMPK antibody (1:1000, CST), rabbit anti-mouse p-AMPK antibody (1:1000, CST), and rabbit anti-mouse CD274 antibody (1:1000, affinity), respectively, at 4 °C overnight. .. After 24 h, they were co-incubated with secondary antibodies at room temperature for another 2 h. Finally, the fluorescence chemiluminescence gel imaging system (Peiqing Science and Technology) was used to obtain the image of protein bands.



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    Image Search Results


    (A) Gene set enrichment analysis (GSEA) plots derived from RNA-Seq analysis of Cas9-expressing HepG2 cells transduced with sgRNA targeting KEAP1 (sg KEAP1 ) versus cells transduced with sgRNA targeting the safe harbour locus AAVS1 (sgAAVS1), n=4. HNF4A signature, Gene Set SUMI_HNF4A_TARGETS; Cholangiocyte signature, Gene set AIZARANI_LIVER_C39_EPCAM_POS_BILE_DUCT_CELLS_4. (B) Representative immunoblot analysis of sgAAVS1 and sg KEAP1 HepG2 cells. (C) GSEA plot derived from RNA-Seq analysis of HepG2 cells harbouring inducible expression of EGFP (Control) versus cells harbouring inducible expression of NRF2 T80K (TO:NRF2 T80K ) after 4 days of DOX-treatment, n=3. NRF2 pathway signature corresponds to Gene Set WP_NRF2_PATHWAY. (D) Volcano plot of differentially expressed genes (DEGs) identified by RNA-Seq analysis of TO:NRF2 T80K versus Control HepG2 cells after 4 days of DOX treatment, n=3. Significant DEGs are highlighted in pink. Select canonical NRF2 target genes are highlighted in blue. Cholangiocyte markers are highlighted in green. (E) GSEA plots derived from RNA-Seq analysis of Control versus TO:NRF2 T80K HepG2 cells, n=3. HNF4A signature, Gene Set SUMI_HNF4A_TARGETS; Cholangiocyte signature, Gene set AIZARANI_LIVER_C39_EPCAM_POS_BILE_DUCT_CELLS_4. (F) Representative immunoblot analysis of Control and TO:NRF2 T80K HepG2 cells after 4 days of DOX treatment. (G) Representative confocal images of hepatocyte membrane ( lf :CFP-CAAX, cyan), cholangiocytes ( anxa4 :mScarlet3, red), and a 3D-rendered mask of the cholangiocyte network derived from TO:NRF2 T80K ; anxa4 :mScarlet3 zebrafish larvae treated with DMSO or DOX from 5 to 10 days post-fertilisation (dpf). White scale bars represent 50 µm. (H) Quantification of mScarlet3 (cholangiocyte) area normalized to CFP (liver) area, n=8. (I) Magnification of liver sections denoted by hashed lines in (G). White arrows indicate cells expressing hepatocyte (cyan) and cholangiocyte (red) markers. Scale bars represent 10 µm.

    Journal: bioRxiv

    Article Title: NRF2 co-opts the SWI/SNF complex to drive liver cell plasticity

    doi: 10.64898/2026.02.09.704724

    Figure Lengend Snippet: (A) Gene set enrichment analysis (GSEA) plots derived from RNA-Seq analysis of Cas9-expressing HepG2 cells transduced with sgRNA targeting KEAP1 (sg KEAP1 ) versus cells transduced with sgRNA targeting the safe harbour locus AAVS1 (sgAAVS1), n=4. HNF4A signature, Gene Set SUMI_HNF4A_TARGETS; Cholangiocyte signature, Gene set AIZARANI_LIVER_C39_EPCAM_POS_BILE_DUCT_CELLS_4. (B) Representative immunoblot analysis of sgAAVS1 and sg KEAP1 HepG2 cells. (C) GSEA plot derived from RNA-Seq analysis of HepG2 cells harbouring inducible expression of EGFP (Control) versus cells harbouring inducible expression of NRF2 T80K (TO:NRF2 T80K ) after 4 days of DOX-treatment, n=3. NRF2 pathway signature corresponds to Gene Set WP_NRF2_PATHWAY. (D) Volcano plot of differentially expressed genes (DEGs) identified by RNA-Seq analysis of TO:NRF2 T80K versus Control HepG2 cells after 4 days of DOX treatment, n=3. Significant DEGs are highlighted in pink. Select canonical NRF2 target genes are highlighted in blue. Cholangiocyte markers are highlighted in green. (E) GSEA plots derived from RNA-Seq analysis of Control versus TO:NRF2 T80K HepG2 cells, n=3. HNF4A signature, Gene Set SUMI_HNF4A_TARGETS; Cholangiocyte signature, Gene set AIZARANI_LIVER_C39_EPCAM_POS_BILE_DUCT_CELLS_4. (F) Representative immunoblot analysis of Control and TO:NRF2 T80K HepG2 cells after 4 days of DOX treatment. (G) Representative confocal images of hepatocyte membrane ( lf :CFP-CAAX, cyan), cholangiocytes ( anxa4 :mScarlet3, red), and a 3D-rendered mask of the cholangiocyte network derived from TO:NRF2 T80K ; anxa4 :mScarlet3 zebrafish larvae treated with DMSO or DOX from 5 to 10 days post-fertilisation (dpf). White scale bars represent 50 µm. (H) Quantification of mScarlet3 (cholangiocyte) area normalized to CFP (liver) area, n=8. (I) Magnification of liver sections denoted by hashed lines in (G). White arrows indicate cells expressing hepatocyte (cyan) and cholangiocyte (red) markers. Scale bars represent 10 µm.

    Article Snippet: Membranes were incubated with anti-HNF4α (C11F12) Rabbit mAb (Cell Signaling Technology, 3113), anti-β-Actin (8H10D10) Mouse mAb (Cell Signaling Technology, 3700), anti-NRF2 (E5F1A) Rabbit mAb (Cell Signaling Technology, 20733), or anti-Histone H3 Rabbit pAb (Abcam, ab1791) primary antibodies prior to incubation with IRDye secondary antibodies (LI-COR) and imaged using the Odyssey DLx imaging system (LI-COR).

    Techniques: Derivative Assay, RNA Sequencing, Expressing, Transduction, Western Blot, Control, Membrane

    (A) Quantitative PCR (qPCR) analysis of GCLM expression in Control and TO:NRF2 T80K HepG2 cells treated with DOX for 4 days, and sgAAVS1 and sg KEAP1 HepG2 cells, n=3. (B) Representative immunoblot analysis of nuclear fractions from Control and TO:NRF2 T80K HepG2 cells treated with 200 ng/mL DOX for 4 days and sgAAVS1 and sg KEAP1 HepG2 cells. (C) Representative confocal images of CFP (hepatocyte, cyan) and EGFP (NRF2 target gene expression, green) from TO:NRF2 T80K ; gstp1: EGFP zebrafish larvae treated with DMSO or DOX from 5 to 6 dpf. Scale bars represent 50 µm. (D) Schematic of the approach employed to generate the TgKI(mScarlet3-P2A-anxa4) transgenic zebrafish line. (E) Representative confocal images of mScarlet3 (cholangiocyte) from anxa4 :mScarlet3 zebrafish larvae at treated with DMSO or DOX from 5 to 10 dpf. Scale bars represent 50 µm. (F) Immunofluorescent staining of Anxa4 (cholangiocyte) in liver sections from WT and keap1a/b crispants at 14 dpf. Scale bars represent 25 µm.

    Journal: bioRxiv

    Article Title: NRF2 co-opts the SWI/SNF complex to drive liver cell plasticity

    doi: 10.64898/2026.02.09.704724

    Figure Lengend Snippet: (A) Quantitative PCR (qPCR) analysis of GCLM expression in Control and TO:NRF2 T80K HepG2 cells treated with DOX for 4 days, and sgAAVS1 and sg KEAP1 HepG2 cells, n=3. (B) Representative immunoblot analysis of nuclear fractions from Control and TO:NRF2 T80K HepG2 cells treated with 200 ng/mL DOX for 4 days and sgAAVS1 and sg KEAP1 HepG2 cells. (C) Representative confocal images of CFP (hepatocyte, cyan) and EGFP (NRF2 target gene expression, green) from TO:NRF2 T80K ; gstp1: EGFP zebrafish larvae treated with DMSO or DOX from 5 to 6 dpf. Scale bars represent 50 µm. (D) Schematic of the approach employed to generate the TgKI(mScarlet3-P2A-anxa4) transgenic zebrafish line. (E) Representative confocal images of mScarlet3 (cholangiocyte) from anxa4 :mScarlet3 zebrafish larvae at treated with DMSO or DOX from 5 to 10 dpf. Scale bars represent 50 µm. (F) Immunofluorescent staining of Anxa4 (cholangiocyte) in liver sections from WT and keap1a/b crispants at 14 dpf. Scale bars represent 25 µm.

    Article Snippet: Membranes were incubated with anti-HNF4α (C11F12) Rabbit mAb (Cell Signaling Technology, 3113), anti-β-Actin (8H10D10) Mouse mAb (Cell Signaling Technology, 3700), anti-NRF2 (E5F1A) Rabbit mAb (Cell Signaling Technology, 20733), or anti-Histone H3 Rabbit pAb (Abcam, ab1791) primary antibodies prior to incubation with IRDye secondary antibodies (LI-COR) and imaged using the Odyssey DLx imaging system (LI-COR).

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Control, Western Blot, Targeted Gene Expression, Transgenic Assay, Staining

    (A) Representative H&E and immunofluorescent staining of CFP (hepatocyte, magenta) and Anxa4 (cholangiocyte, yellow) in liver sections from adult TO:NRF2 T80K zebrafish after 4 weeks treatment with DMSO or DOX. Scale bars represent 50 µm. (B) Magnification of liver sections denoted by hashed lines in (A). White arrows indicate cells that express CFP (hepatocyte, magenta) and Anxa4 (cholangiocyte, yellow) markers. Scale bars represent 10 µm. (C) Heatmap of hepatocyte and cholangiocyte gene expression in livers from adult TO:NRF2 T80K zebrafish after 4 weeks treatment with DMSO or DOX as determined by bulk RNA-Seq analysis, n=3. (D) Uniform Manifold Approximation and Projection (UMAP) visualization of scRNA-Seq data obtained from livers from adult TO:NRF2 T80K zebrafish after 3 weeks treatment with DMSO or DOX. (E) UMAP visualization from the joint clustering of scRNA-Seq data obtained from livers from adult TO:NRF2 T80K zebrafish after 3 weeks treatment with DMSO or DOX. Chol: cholangiocytes; ECs: Endothelial cells; Ery: Erythrocytes; Hep: Hepatocytes; HS_Cells: Hepatic stellate cells; Lymph: Lymphocytes; Mac: Macrophages; Nrf2_trans: Transdifferentiating cells; GB_Cells: Gall bladder cells. (F) Stacked bar chart showing the relative proportions of the major cell types in livers from adult TO:NRF2 T80K zebrafish treated with DMSO or DOX for 3 weeks. (G) UMAP visualization of fabp10a (hepatocyte) and anxa4 (cholangiocyte) expression in livers from adult TO:NRF2 T80K zebrafish treated with DMSO or DOX for 3 weeks. Hashed boxes outline cells expressing both markers.

    Journal: bioRxiv

    Article Title: NRF2 co-opts the SWI/SNF complex to drive liver cell plasticity

    doi: 10.64898/2026.02.09.704724

    Figure Lengend Snippet: (A) Representative H&E and immunofluorescent staining of CFP (hepatocyte, magenta) and Anxa4 (cholangiocyte, yellow) in liver sections from adult TO:NRF2 T80K zebrafish after 4 weeks treatment with DMSO or DOX. Scale bars represent 50 µm. (B) Magnification of liver sections denoted by hashed lines in (A). White arrows indicate cells that express CFP (hepatocyte, magenta) and Anxa4 (cholangiocyte, yellow) markers. Scale bars represent 10 µm. (C) Heatmap of hepatocyte and cholangiocyte gene expression in livers from adult TO:NRF2 T80K zebrafish after 4 weeks treatment with DMSO or DOX as determined by bulk RNA-Seq analysis, n=3. (D) Uniform Manifold Approximation and Projection (UMAP) visualization of scRNA-Seq data obtained from livers from adult TO:NRF2 T80K zebrafish after 3 weeks treatment with DMSO or DOX. (E) UMAP visualization from the joint clustering of scRNA-Seq data obtained from livers from adult TO:NRF2 T80K zebrafish after 3 weeks treatment with DMSO or DOX. Chol: cholangiocytes; ECs: Endothelial cells; Ery: Erythrocytes; Hep: Hepatocytes; HS_Cells: Hepatic stellate cells; Lymph: Lymphocytes; Mac: Macrophages; Nrf2_trans: Transdifferentiating cells; GB_Cells: Gall bladder cells. (F) Stacked bar chart showing the relative proportions of the major cell types in livers from adult TO:NRF2 T80K zebrafish treated with DMSO or DOX for 3 weeks. (G) UMAP visualization of fabp10a (hepatocyte) and anxa4 (cholangiocyte) expression in livers from adult TO:NRF2 T80K zebrafish treated with DMSO or DOX for 3 weeks. Hashed boxes outline cells expressing both markers.

    Article Snippet: Membranes were incubated with anti-HNF4α (C11F12) Rabbit mAb (Cell Signaling Technology, 3113), anti-β-Actin (8H10D10) Mouse mAb (Cell Signaling Technology, 3700), anti-NRF2 (E5F1A) Rabbit mAb (Cell Signaling Technology, 20733), or anti-Histone H3 Rabbit pAb (Abcam, ab1791) primary antibodies prior to incubation with IRDye secondary antibodies (LI-COR) and imaged using the Odyssey DLx imaging system (LI-COR).

    Techniques: Staining, Gene Expression, RNA Sequencing, Expressing

    (A) Volcano plot of DEGs identified by bulk RNA-Seq analysis of livers from adult TO:NRF2 T80K zebrafish treated with DOX versus DMSO for 4 weeks, n=3. Significant DEGs are highlighted in pink. Select canonical NRF2 target genes are highlighted in blue. (B) UMAP visualization of gstp1 expression in livers from adult TO:NRF2 T80K zebrafish treated with DMSO or DOX for 3 weeks. (C) Masson’s trichome stained histological sections from livers isolated from adult TO:NRF2 T80K zebrafish after 4 weeks treatment with DMSO or DOX. Scale bars represent 50 µm. (D) TUNEL- and DAPI-stained histological sections from livers isolated from adult TO:NRF2 T80K zebrafish after 4 weeks treatment with DMSO or DOX. DNase I treatment was employed as a positive control. Scale bars represent 50 µm.

    Journal: bioRxiv

    Article Title: NRF2 co-opts the SWI/SNF complex to drive liver cell plasticity

    doi: 10.64898/2026.02.09.704724

    Figure Lengend Snippet: (A) Volcano plot of DEGs identified by bulk RNA-Seq analysis of livers from adult TO:NRF2 T80K zebrafish treated with DOX versus DMSO for 4 weeks, n=3. Significant DEGs are highlighted in pink. Select canonical NRF2 target genes are highlighted in blue. (B) UMAP visualization of gstp1 expression in livers from adult TO:NRF2 T80K zebrafish treated with DMSO or DOX for 3 weeks. (C) Masson’s trichome stained histological sections from livers isolated from adult TO:NRF2 T80K zebrafish after 4 weeks treatment with DMSO or DOX. Scale bars represent 50 µm. (D) TUNEL- and DAPI-stained histological sections from livers isolated from adult TO:NRF2 T80K zebrafish after 4 weeks treatment with DMSO or DOX. DNase I treatment was employed as a positive control. Scale bars represent 50 µm.

    Article Snippet: Membranes were incubated with anti-HNF4α (C11F12) Rabbit mAb (Cell Signaling Technology, 3113), anti-β-Actin (8H10D10) Mouse mAb (Cell Signaling Technology, 3700), anti-NRF2 (E5F1A) Rabbit mAb (Cell Signaling Technology, 20733), or anti-Histone H3 Rabbit pAb (Abcam, ab1791) primary antibodies prior to incubation with IRDye secondary antibodies (LI-COR) and imaged using the Odyssey DLx imaging system (LI-COR).

    Techniques: RNA Sequencing, Expressing, Staining, Isolation, TUNEL Assay, Positive Control

    (A) Schematic of DMSO/DOX pulse-chase experiments in adult TO:NRF2 T80K zebrafish. (B) Representative H&E-staining in liver sections from adult TO:NRF2 T80K zebrafish after 7- or 14-days treatment with either DMSO or DOX, followed by 7- or 28-days withdrawal of DMSO or DOX. Scale bars represent 50 µm. (C) Representative immunofluorescent staining of CFP (hepatocyte, magenta) and Anxa4 (cholangiocyte, yellow) in liver sections from adult TO:NRF2 T80K zebrafish after 7- or 14-days treatment with either DMSO or DOX, followed by 7- or 28-days withdrawal of DMSO or DOX. Scale bars represent 50 µm. (D) Multidimensional scaling (MDS) plot of bulk RNA-Seq data obtained from livers isolated from adult TO:NRF2 T80K zebrafish after 14 days treatment with either DMSO or DOX, followed by 28-days withdrawal of DMSO or DOX, n=3. (E) Heatmap of hepatocyte and cholangiocyte gene expression in livers isolated from adult TO:NRF2 T80K zebrafish after 7- or 14-days treatment with either DMSO or DOX, followed by 7- or 28-days withdrawal of DMSO or DOX, as determined by bulk RNA-Seq analysis, n=3. (F) Volcano plot of DEGs identified by bulk RNA-Seq analysis of livers isolated from adult TO:NRF2 T80K zebrafish treated with DOX versus DMSO for 14 days (left), followed by withdrawal of DOX versus DMSO for 28 days (right), n=3. Significant DEGs are highlighted in pink. Hepatocyte markers are highlighted in blue. Cholangiocyte markers are highlighted in green. (G) Volcano plot of DEGs identified by RNA-Seq analysis of TO:NRF2 T80K versus Control HepG2 cells after 4 days of DOX treatment (left), followed by withdrawal of DOX for 4 days (right). Significant DEGs are highlighted in pink. Hepatocyte markers are highlighted in blue. Cholangiocyte markers are highlighted in green.

    Journal: bioRxiv

    Article Title: NRF2 co-opts the SWI/SNF complex to drive liver cell plasticity

    doi: 10.64898/2026.02.09.704724

    Figure Lengend Snippet: (A) Schematic of DMSO/DOX pulse-chase experiments in adult TO:NRF2 T80K zebrafish. (B) Representative H&E-staining in liver sections from adult TO:NRF2 T80K zebrafish after 7- or 14-days treatment with either DMSO or DOX, followed by 7- or 28-days withdrawal of DMSO or DOX. Scale bars represent 50 µm. (C) Representative immunofluorescent staining of CFP (hepatocyte, magenta) and Anxa4 (cholangiocyte, yellow) in liver sections from adult TO:NRF2 T80K zebrafish after 7- or 14-days treatment with either DMSO or DOX, followed by 7- or 28-days withdrawal of DMSO or DOX. Scale bars represent 50 µm. (D) Multidimensional scaling (MDS) plot of bulk RNA-Seq data obtained from livers isolated from adult TO:NRF2 T80K zebrafish after 14 days treatment with either DMSO or DOX, followed by 28-days withdrawal of DMSO or DOX, n=3. (E) Heatmap of hepatocyte and cholangiocyte gene expression in livers isolated from adult TO:NRF2 T80K zebrafish after 7- or 14-days treatment with either DMSO or DOX, followed by 7- or 28-days withdrawal of DMSO or DOX, as determined by bulk RNA-Seq analysis, n=3. (F) Volcano plot of DEGs identified by bulk RNA-Seq analysis of livers isolated from adult TO:NRF2 T80K zebrafish treated with DOX versus DMSO for 14 days (left), followed by withdrawal of DOX versus DMSO for 28 days (right), n=3. Significant DEGs are highlighted in pink. Hepatocyte markers are highlighted in blue. Cholangiocyte markers are highlighted in green. (G) Volcano plot of DEGs identified by RNA-Seq analysis of TO:NRF2 T80K versus Control HepG2 cells after 4 days of DOX treatment (left), followed by withdrawal of DOX for 4 days (right). Significant DEGs are highlighted in pink. Hepatocyte markers are highlighted in blue. Cholangiocyte markers are highlighted in green.

    Article Snippet: Membranes were incubated with anti-HNF4α (C11F12) Rabbit mAb (Cell Signaling Technology, 3113), anti-β-Actin (8H10D10) Mouse mAb (Cell Signaling Technology, 3700), anti-NRF2 (E5F1A) Rabbit mAb (Cell Signaling Technology, 20733), or anti-Histone H3 Rabbit pAb (Abcam, ab1791) primary antibodies prior to incubation with IRDye secondary antibodies (LI-COR) and imaged using the Odyssey DLx imaging system (LI-COR).

    Techniques: Pulse Chase, Staining, RNA Sequencing, Isolation, Gene Expression, Control

    (A) Periodic Acid-Schiff (PAS)-stained histological sections from livers isolated from adult TO:NRF2 T80K zebrafish after 7- or 14-days treatment with DMSO or DOX, followed by 7- or 28-days withdrawal of DMSO or DOX. Scale bars represent 50 µm. (B) Heatmap of NRF2 target gene expression in livers isolated from adult TO:NRF2 T80K zebrafish after 7- or 14-days treatment with DMSO or DOX, followed by 7- or 28-days withdrawal of DMSO or DOX as determined by bulk RNA-Seq analysis, n=3. (C) MDS Plot of RNA-Seq data from Control and TO:NRF2 T80K HepG2 cells after 4 days of DOX treatment, followed by withdrawal of DOX for 4 days.

    Journal: bioRxiv

    Article Title: NRF2 co-opts the SWI/SNF complex to drive liver cell plasticity

    doi: 10.64898/2026.02.09.704724

    Figure Lengend Snippet: (A) Periodic Acid-Schiff (PAS)-stained histological sections from livers isolated from adult TO:NRF2 T80K zebrafish after 7- or 14-days treatment with DMSO or DOX, followed by 7- or 28-days withdrawal of DMSO or DOX. Scale bars represent 50 µm. (B) Heatmap of NRF2 target gene expression in livers isolated from adult TO:NRF2 T80K zebrafish after 7- or 14-days treatment with DMSO or DOX, followed by 7- or 28-days withdrawal of DMSO or DOX as determined by bulk RNA-Seq analysis, n=3. (C) MDS Plot of RNA-Seq data from Control and TO:NRF2 T80K HepG2 cells after 4 days of DOX treatment, followed by withdrawal of DOX for 4 days.

    Article Snippet: Membranes were incubated with anti-HNF4α (C11F12) Rabbit mAb (Cell Signaling Technology, 3113), anti-β-Actin (8H10D10) Mouse mAb (Cell Signaling Technology, 3700), anti-NRF2 (E5F1A) Rabbit mAb (Cell Signaling Technology, 20733), or anti-Histone H3 Rabbit pAb (Abcam, ab1791) primary antibodies prior to incubation with IRDye secondary antibodies (LI-COR) and imaged using the Odyssey DLx imaging system (LI-COR).

    Techniques: Staining, Isolation, Targeted Gene Expression, RNA Sequencing, Control

    (A) Schematic of the epigenetic-focused small molecule inhibitor screen. (B) Heatmap of NRF2 target gene expression in livers isolated from adult TO:NRF2 T80K zebrafish treated with DMSO or DOX in the absence or presence of 250 nM FHD-286 for 14 days as determined by bulk RNA-Seq analysis, n=3. (C) Volcano plot of DEGs identified by bulk RNA-Seq of livers isolated from adult TO:NRF2 T80K zebrafish treated with DOX versus DMSO both in the presence of 250 nM FHD-286 for 14 days as determined by bulk RNA-Seq analysis, n=3. Significant DEGs are highlighted in pink. Select canonical NRF2 target genes are highlighted in blue.

    Journal: bioRxiv

    Article Title: NRF2 co-opts the SWI/SNF complex to drive liver cell plasticity

    doi: 10.64898/2026.02.09.704724

    Figure Lengend Snippet: (A) Schematic of the epigenetic-focused small molecule inhibitor screen. (B) Heatmap of NRF2 target gene expression in livers isolated from adult TO:NRF2 T80K zebrafish treated with DMSO or DOX in the absence or presence of 250 nM FHD-286 for 14 days as determined by bulk RNA-Seq analysis, n=3. (C) Volcano plot of DEGs identified by bulk RNA-Seq of livers isolated from adult TO:NRF2 T80K zebrafish treated with DOX versus DMSO both in the presence of 250 nM FHD-286 for 14 days as determined by bulk RNA-Seq analysis, n=3. Significant DEGs are highlighted in pink. Select canonical NRF2 target genes are highlighted in blue.

    Article Snippet: Membranes were incubated with anti-HNF4α (C11F12) Rabbit mAb (Cell Signaling Technology, 3113), anti-β-Actin (8H10D10) Mouse mAb (Cell Signaling Technology, 3700), anti-NRF2 (E5F1A) Rabbit mAb (Cell Signaling Technology, 20733), or anti-Histone H3 Rabbit pAb (Abcam, ab1791) primary antibodies prior to incubation with IRDye secondary antibodies (LI-COR) and imaged using the Odyssey DLx imaging system (LI-COR).

    Techniques: Targeted Gene Expression, Isolation, RNA Sequencing

    (A) Quantification of cholangiocyte area (mScarlet3), as a ratio of liver area (CFP) area, from an in vivo chemical suppressor screen using an epigenetic-focused library of small molecule inhibitors. The blue dashed line indicates the mean of the DMSO-treated group, whereas the red dashed line indicates the mean of the DOX-treated group. (B) Representative confocal images of CFP (hepatocyte, cyan), mScarlet3 (cholangiocyte, red), and a 3D-rendered mask of the cholangiocyte network from TO:NRF2 T80K ; anxa4 :mScarlet3 zebrafish larvae with DMSO or DOX in the absence or presence of 100 nM FHD-286 from 5 to 10 dpf. White scale bars represent 50 µm. (C) Quantification of mScarlet3 (cholangiocyte) area normalized to CFP (liver) area, n=11. (D) Representative H&E and immunofluorescent staining of CFP (hepatocyte, magenta) and Anxa4 (cholangiocyte, yellow) in liver sections from adult TO:NRF2 T80K zebrafish treated with DMSO or DOX in the absence or presence of 250 nM FHD-286 for 14 days. (E) Quantification of Anxa4 (cholangiocyte) area normalized to CFP (liver) area, n=6. (F) Volcano plot of DEGs identified by bulk RNA-Seq analysis of livers isolated from adult TO:NRF2 T80K zebrafish treated with DOX (left) or DMSO (right) in the presence versus absence of 250 nM FHD-286 for 14 days. Significant DEGs are highlighted in pink. Hepatocyte markers are highlighted in blue. Cholangiocyte markers are highlighted in green. (G) Gene essentiality scores for SMARCA4 (left) and SMARCA2 (right) in KEAP1 (WT) and KEAP1 (Mutant) samples from TCGA DEPMAP .

    Journal: bioRxiv

    Article Title: NRF2 co-opts the SWI/SNF complex to drive liver cell plasticity

    doi: 10.64898/2026.02.09.704724

    Figure Lengend Snippet: (A) Quantification of cholangiocyte area (mScarlet3), as a ratio of liver area (CFP) area, from an in vivo chemical suppressor screen using an epigenetic-focused library of small molecule inhibitors. The blue dashed line indicates the mean of the DMSO-treated group, whereas the red dashed line indicates the mean of the DOX-treated group. (B) Representative confocal images of CFP (hepatocyte, cyan), mScarlet3 (cholangiocyte, red), and a 3D-rendered mask of the cholangiocyte network from TO:NRF2 T80K ; anxa4 :mScarlet3 zebrafish larvae with DMSO or DOX in the absence or presence of 100 nM FHD-286 from 5 to 10 dpf. White scale bars represent 50 µm. (C) Quantification of mScarlet3 (cholangiocyte) area normalized to CFP (liver) area, n=11. (D) Representative H&E and immunofluorescent staining of CFP (hepatocyte, magenta) and Anxa4 (cholangiocyte, yellow) in liver sections from adult TO:NRF2 T80K zebrafish treated with DMSO or DOX in the absence or presence of 250 nM FHD-286 for 14 days. (E) Quantification of Anxa4 (cholangiocyte) area normalized to CFP (liver) area, n=6. (F) Volcano plot of DEGs identified by bulk RNA-Seq analysis of livers isolated from adult TO:NRF2 T80K zebrafish treated with DOX (left) or DMSO (right) in the presence versus absence of 250 nM FHD-286 for 14 days. Significant DEGs are highlighted in pink. Hepatocyte markers are highlighted in blue. Cholangiocyte markers are highlighted in green. (G) Gene essentiality scores for SMARCA4 (left) and SMARCA2 (right) in KEAP1 (WT) and KEAP1 (Mutant) samples from TCGA DEPMAP .

    Article Snippet: Membranes were incubated with anti-HNF4α (C11F12) Rabbit mAb (Cell Signaling Technology, 3113), anti-β-Actin (8H10D10) Mouse mAb (Cell Signaling Technology, 3700), anti-NRF2 (E5F1A) Rabbit mAb (Cell Signaling Technology, 20733), or anti-Histone H3 Rabbit pAb (Abcam, ab1791) primary antibodies prior to incubation with IRDye secondary antibodies (LI-COR) and imaged using the Odyssey DLx imaging system (LI-COR).

    Techniques: In Vivo, Staining, RNA Sequencing, Isolation, Mutagenesis